Composite

Part:BBa_K3237024:Design

Designed by: Luke Saville, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-09)


NAB1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 1565
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1535
    Illegal NheI site found at 1087
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
    Illegal NotI site found at 1571
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1535
    Illegal BamHI site found at 1553
    Illegal XhoI site found at 577
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 1565
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 1565
    Illegal SpeI site found at 1559
    Illegal PstI site found at 1073
    Illegal PstI site found at 1541
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NAB1 protein was codon optimized for use in C. reinhardtii .

Source

NAB1 sequence is based off a sequence attained from the NCBI database accession number EDP08495.1. The part was synthesized by idt.


References

1. Mussgnug, J., Wobbe, L., Elles, I., Claus, C., Hamilton, M., Fink, A., Kahmann, U., Kapazoglou, A., Mullineaux, C., Hippler, M., Nickelsen, J., Nixon, P., and Kruse, O. (2005) NAB1 Is an RNA Binding Protein Involved in theLight-Regulated Differential Expression of theLight-Harvesting Antenna ofChlamydomonas reinhardtii. The plant cell. 17, 3409-3421

2. Beckmann, J., lehr, F., Finazzi, G., Kankamer, B., Posten, C., Wobbe, L., and Kruse, O. (2009) Improvement of light to biomass conversion by de-regulation of light-harvesting protein translation in Chlamydomonas reinhardtii. Journal of Biotechnology. 142, 70-77